TopQ miRNA Probe qPCR Assay
602001
| PN | Product Name | Size | Price (TWD) |
| 602001 | TopQ miRNA Probe qPCR Assay |
250ul (500rxns) |
8,400 |
| 組分 | |
| 20X TaqMan miRNA Assay | 250 µl (500 rxns) |
| 20X miRNA RT Primer | 200 µl (200 rxns) |
| miRNA Positive Control | 100 µl (1E5 Copies/µl) |
● 市面上唯一提供miRNA標準品 確保實驗準確無誤!
● 實驗特點:
- Step 1 : Reverse Transcription
- Step 2 : Real-time PCR reaction
庫存清單
|
Human
|
Others
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|---|---|
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hsa-let-7a-5p
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cel-miR-39-3p
|
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hsa-let-7b-5p
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mmu-miR-350-3p
|
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hsa-miR-101-3p
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mmu-miR-24-2-5p
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hsa-miR-10b-5p
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U6 snRNA
|
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hsa-miR-122-5p
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mmu-miR-3535
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hsa-miR-1246
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mmu-miR-152-3p
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hsa-miR-125b-5p
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mmu-miR-24-3p
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hsa-miR-126-3p
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mmu-miR-129-5p
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hsa-miR-126-5p
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mmu-let-7k
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hsa-miR-1290
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hsa-miR-132-3p
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hsa-miR-136-3p
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hsa-miR-137-3p
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hsa-miR-140-5p
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hsa-miR-142-3p
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hsa-miR-145-5p
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hsa-miR-146a-5p
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hsa-miR-148a-3p
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hsa-miR-149-5p
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|
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hsa-miR-15a-5p
|
|
| hsa-miR-155-5p | |
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hsa-miR-16-5p
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hsa-miR-17-3p
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hsa-miR-17-5p
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hsa-miR-181a-5p
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hsa-miR-181b-5p
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hsa-miR-181c-5p
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hsa-miR-182-3p
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|
| hsa-miR-184 | |
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hsa-miR-187-3p
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hsa-miR-18a-5p
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hsa-miR-191-5p
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hsa-miR-192-5p
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hsa-miR-193a-5p
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hsa-miR-194-3p
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hsa-miR-194-5p
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hsa-miR-195-5p
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hsa-miR-196b-3p
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hsa-miR-196b-5p
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hsa-miR-1972
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hsa-miR-199a-5p
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hsa-miR-19a-3p
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hsa-miR-19b-3p
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hsa-miR-200b-3p
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hsa-miR-200c-3p
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hsa-miR-20a-5p
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hsa-miR-210-3p
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hsa-miR-211-5p
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hsa-miR-212-3p
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hsa-miR-214-3p
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hsa-miR-21-5p
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hsa-miR-218-5p
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hsa-miR-221-5p
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hsa-miR-222-3p
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hsa-miR-23b-3p
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hsa-miR-26a-5p
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hsa-miR-27b-3p
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hsa-miR-296-3p
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hsa-miR-29a-3p
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hsa-miR-29a-5p
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hsa-miR-30c-5p
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hsa-miR-30d-5p
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hsa-miR-30e-5p
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hsa-miR-31-5p
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hsa-miR-3177-5p
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hsa-miR-3194-5p
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hsa-miR-320a-3p
|
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hsa-miR-330-3p
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hsa-miR-3620-5p
|
|
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hsa-miR-363-5p
|
|
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hsa-miR-3663-3p
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hsa-miR-370-3p
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hsa-miR-373-3p
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hsa-miR-374b-5p
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hsa-miR-375-3p
|
|
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hsa-miR-379-5p
|
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hsa-miR-409-5p
|
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hsa-miR-423-5p
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hsa-miR-425-5p
|
|
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hsa-miR-449a
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hsa-miR-4652-5p
|
|
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hsa-miR-4669
|
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hsa-miR-483-3p
|
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hsa-miR-486-5p
|
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hsa-miR-487b-3p
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hsa-miR-491-5p
|
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hsa-miR-495-3p
|
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hsa-miR-5010-3p
|
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hsa-miR-502-5p
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hsa-miR-516a-5p
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hsa-miR-518d-3p
|
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hsa-miR-519a-3p
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hsa-miR-520b-3p
|
|
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hsa-miR-524-5p
|
|
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hsa-miR-532-5p
|
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hsa-miR-539-5p
|
|
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hsa-miR-545-3p
|
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hsa-miR-548ah-3p
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hsa-miR-548av-3p
|
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hsa-miR-6503-3p
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hsa-miR-652-5p
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hsa-miR-7-1-3p
|
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hsa-miR-7-5p
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hsa-miR-92a-3p
|
|
| hsa-miR-148b-3p | |
| hsa-miR-147b-3p | |
| hsa-miR-372-3p | |
| hsa-miR-196a-5p | |
| hsa-miR-10a-5p | |
| hsa-miR-335-3p | |
| hsa-miR-543 | |
| hsa-miR-33b-5p | |
| hsa-miR-331-3p | |
| hsa-miR-361-5p | |
| hsa-miR-485-3p | |
| hsa-let-7c-5p | |
| hsa-miR-1-3p | |
| hsa-miR-134-5p | |
| hsa-miR-361-5p | |
| hsa-miR-409-3p | |
| hsa-miR-193b-3p | |
| hsa-miR-125a-5p | |
| hsa-miR-221-3p | |
| hsa-miR-494-5p | |
| hsa-miR-149-3p | |
| hsa-miR-11181-5p | |
| hsa-miR-589-5p | |
| hsa-miR-18a-5p |
說明
● microRNA為19-22nt的短的非编碼RNA。
● 與mRNA的3'-UTR區域結合，進而在轉錄後調控mRNA的表達。
● 在不同物種中，miRNA 序列具有高度的保守性 ( Conserved )，具有組織特異性。
● microRNA的表達改變與目前各種已知疾病相關。
原理
● 採用廣泛認可的Stem-loop法，其包含miRNA特異反轉錄引物以及qPCR正、反向引子。
環結構狀的miRNA特異反轉錄引物可特異性的轉錄mature miRNA。再利用高度特異的正反向引子，使miRNA定量既靈敏又具高度特異。
● 提供miRNA spike-in，以監控miRNA純化過程是否有較大損失和反轉錄與定量PCR是否有PCR抑制劑。
實驗範例
miRNA檢測範例
圖1. qPCR檢測MCF Cell 中miRNA-21-5p的表達情況
*miRBase連結: www.mirbase.org/
| NO. | miRNA | Accession | Species | 備註 |
| 0 | hsa-miR-21-5p | MIMAT0000076 | Human | 範例 |
| 1 | ||||
| 2 |
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