TopQ miRNA Probe qPCR Assay

602001
　
 
 
PN Product Name Size Price (TWD)
602001 TopQ miRNA Probe
qPCR Assay		 250ul
(500rxns)		 8,400
 
組分  
20X TaqMan miRNA Assay 250 µl (500 rxns)
20X miRNA RT Primer 200 µl (200 rxns)
miRNA Positive Control 100 µl (1E5 Copies/µl)
   

 
  產品特點   

● 市面上唯一提供miRNA標準品 確保實驗準確無誤!
● 實驗特點:
     - Step 1 : Reverse Transcription
 

     - Step 2 : Real-time PCR reaction



  庫存清單     
 
Human
hsa-let-7a-3p
hsa-let-7a-5p
hsa-let-7b-5p
hsa-let-7c-5p
hsa-let-7i-5p
hsa-miR-101-3p
hsa-miR-10a-5p
hsa-miR-10b-5p
hsa-miR-11181-5p
hsa-miR-122-5p
hsa-miR-1246
hsa-miR-125a-5p
hsa-miR-125b-5p
hsa-miR-126-3p
hsa-miR-126-5p
hsa-miR-1290
hsa-miR-130a-5p
hsa-miR-132-3p
hsa-miR-133a-3p
hsa-miR-133b
hsa-miR-134-5p
hsa-miR-136-3p
hsa-miR-137-3p
hsa-miR-140-5p
hsa-miR-141-3p
hsa-miR-142-3p
hsa-miR-143-3p
hsa-miR-145-5p
hsa-miR-146a-5p
hsa-miR-146b-5p
hsa-miR-147b-3p
hsa-miR-148a-3p
hsa-miR-148b-3p
hsa-miR-149-3p
hsa-miR-149-5p
hsa-miR-155-5p
hsa-miR-15a-5p
hsa-miR-16-5p
hsa-miR-17-3p
hsa-miR-17-5p
hsa-miR-181a-5p
hsa-miR-181b-5p
hsa-miR-181c-5p
hsa-miR-182-3p
hsa-miR-182-5p
hsa-miR-183-5p
hsa-miR-184
hsa-miR-187-3p
hsa-miR-18a-5p
hsa-miR-191-5p
hsa-miR-192-5p
hsa-miR-193a-5p
hsa-miR-193b-3p
hsa-miR-194-3p
hsa-miR-194-5p
hsa-miR-195-5p
hsa-miR-196a-5p
hsa-miR-196b-3p
hsa-miR-196b-5p
hsa-miR-1972
hsa-miR-199a-3p
hsa-miR-199a-5p
hsa-miR-19a-3p
hsa-miR-19b-3p
hsa-miR-1-3p
hsa-miR-200a-3p
hsa-miR-200b-3p
hsa-miR-200c-3p
hsa-miR-206
hsa-miR-20a-5p
hsa-miR-210-3p
hsa-miR-211-5p
hsa-miR-212-3p
hsa-miR-214-3p
hsa-miR-216a-5p
hsa-miR-21-5p
hsa-miR-218-5p
hsa-miR-221-3p
hsa-miR-221-5p
hsa-miR-222-3p
hsa-miR-22-3p
hsa-miR-224-5p
hsa-miR-23a-3p
hsa-miR-23b-3p
hsa-miR-26a-5p
hsa-miR-27b-3p
hsa-miR-296-3p
hsa-miR-299-5p
hsa-miR-29a-3p
hsa-miR-29a-5p
hsa-miR-29b-3p
hsa-miR-29c-3p
hsa-miR-301a-3p
hsa-miR-301b-3p
hsa-miR-3059-3p
hsa-miR-30a-5p
hsa-miR-30c-5p
hsa-miR-30d-5p
hsa-miR-30e-3p
hsa-miR-30e-5p
hsa-miR-31-3p
hsa-miR-31-5p
hsa-miR-3130-5p
hsa-miR-3177-5p
hsa-miR-3194-5p
hsa-miR-320a-3p
hsa-miR-320d
hsa-miR-330-3p
hsa-miR-331-3p
hsa-miR-335-3p
hsa-miR-33b-5p
hsa-miR-342-3p
hsa-miR-34a-5p
hsa-miR-34c-5p
hsa-miR-361-5p
hsa-miR-3620-5p
hsa-miR-363-5p
hsa-miR-3663-3p
hsa-miR-370-3p
hsa-miR-372-3p
hsa-miR-373-3p
hsa-miR-374b-5p
hsa-miR-375-3p
hsa-miR-376a-3p
hsa-miR-378a-3p
hsa-miR-379-5p
hsa-miR-409-3p
hsa-miR-409-5p
hsa-miR-423-5p
hsa-miR-425-5p
hsa-miR-4443
hsa-miR-4454
hsa-miR-449a
hsa-miR-451a
hsa-miR-4516
hsa-miR-4652-5p
hsa-miR-4669
hsa-miR-483-3p
hsa-miR-485-3p
hsa-miR-483-5p
hsa-miR-486-5p
hsa-miR-487b-3p
hsa-miR-491-5p
hsa-miR-494-5p
hsa-miR-495-3p
hsa-miR-5010-3p
hsa-miR-502-5p
hsa-miR-505-3p
hsa-miR-516a-5p
hsa-miR-518d-3p
hsa-miR-519a-3p
hsa-miR-520b-3p
hsa-miR-524-5p
hsa-miR-532-5p
hsa-miR-539-5p
hsa-miR-543
hsa-miR-545-3p
hsa-miR-548ah-3p
hsa-miR-548av-3p
hsa-miR-589-5p
hsa-miR-6503-3p
hsa-miR-652-5p
hsa-miR-675-5p
hsa-miR-708-3p
hsa-miR-7-1-3p
hsa-miR-7-5p
hsa-miR-92a-3p
hsa-miR-92b-3p
hsa-miR-96-5p
 
Others
cel-miR-39-3p
mmu-let-7k
mmu-miR-1195
mmu-miR-1198-5p
mmu-miR-124-3p
mmu-miR-129-5p
mmu-miR-144-5p
rno-miR-146b-5p
mmu-miR-152-3p
mmu-miR-155-5p
mmu-miR-204-5p
mmu-miR-211-5p
mmu-miR-223-3p
mmu-miR-24-2-5p
mmu-miR-24-3p
mmu-miR-34a-5p
mmu-miR-350-3p
mmu-miR-3535
mmu-miR-362-3p
mmu-miR-434-3p
mmu-miR-483-3p
mmu-miR-505-3p
rno-miR-146b-5p
rno-miR-223-3p
rno-miR-324-3p
rno-miR-3584-5p
rno-miR-378b
rno-miR-93-5p
U6 snRNA (Human/ Mouse/ Rat)
4.5s RNA (Mouse/ Rat)



    說明   

●  microRNA為19-22nt的短的非编碼RNA。
●  與mRNA的3'-UTR區域結合，進而在轉錄後調控mRNA的表達。
●  在不同物種中，miRNA 序列具有高度的保守性 ( Conserved )，具有組織特異性。
●  microRNA的表達改變與目前各種已知疾病相關。

  原理   

●  採用廣泛認可的Stem-loop法，其包含miRNA特異反轉錄引物以及qPCR正、反向引子。
      環結構狀的miRNA特異反轉錄引物可特異性的轉錄mature miRNA。再利用高度特異的正反向引子，使miRNA定量既靈敏又具高度特異。
●  提供miRNA spike-in，以監控miRNA純化過程是否有較大損失和反轉錄與定量PCR是否有PCR抑制劑。




  實驗範例   
miRNA檢測範例



圖1. qPCR檢測MCF Cell 中miRNA-21-5p的表達情況

   訂購方式   


*miRBase連結: www.mirbase.org/
 
NO. miRNA Accession Species 備註
0 hsa-miR-21-5p MIMAT0000076 Human 範例
1 　 　 　 　
2 　 　 　 　
 
 
   相關產品   

• TopGE Probe Fast qPCR Master Mix 更多資訊
• miRscript RT Kit 更多資訊
 
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