(R212) HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper)
R212
| PN | Product Name | Size | Price (TWD) |
| R212-02 | HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper) |
100 rxn (20μl/rxn) |
Inquiry |
優勢
• 高效HiScript II Reverse Transcriptase，超強溫度耐受力在高溫條件下能打開RNA複雜二級結構，得到更長cDNA
• 寬廣的模板起始量範圍，從1 pg-5 μg總RNA，可擴增片段長達15 kb以上
• 含 Random hexamers 與 Anchored Oligo-dT，特異性高，保證第一鏈cDNA合成效率和成功率
• 寬廣的模板起始量範圍，從1 pg-5 μg總RNA，可擴增片段長達15 kb以上
• 含 Random hexamers 與 Anchored Oligo-dT，特異性高，保證第一鏈cDNA合成效率和成功率
• 針對RT-PCR及RT-qPCR均有不同的操作步驟可供選擇
• 檢測靈敏度達 1 pg
• gDNA wiper Mix可迅速徹底去除基因組污染
• 適用stem-loop miRNA cDNA合成 (加入stem-loop miRNA RT primer，不使用hexamers 與 Oligo-dT)
• 檢測靈敏度達 1 pg
• gDNA wiper Mix可迅速徹底去除基因組污染
• 適用stem-loop miRNA cDNA合成 (加入stem-loop miRNA RT primer，不使用hexamers 與 Oligo-dT)
說明
本產品是使用HiScript II Reverse Transcriptase 從Total RNA或Poly (A) + mRNA 合成一鏈cDNA 的試劑盒，含有一鏈cDNA合成所需的全部試劑，HiScript II Reverse Transcriptase 具有更高的cDNA 合成效率及範本的雜質耐受度，更加適合具有複雜二級結構或低豐度的RNA 範本的逆轉錄。Anchored Oligo(dT) 23 VN設計結合位點錨定，特異性高，保證第一鏈cDNA 合成效率和成功率。產品中的4 × gDNA wiper 可在逆轉錄步驟前快速徹底去除基因組污染，提高實驗結果的可信度。
實驗範例
• gDNA wiper快速去除基因組原理說明。
論文發表
• Liu X ,Wei W ,Li X , et al. (2017). BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3.Gastroenterology. IF:18.392
•Ju J, Chen A, Deng Y, et al. (2017).NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression.[J]. Nature Communications, 2017, 8(1):928. IF:12.124
•Ding W, Liu WQ, Jia Y, et al. (2016). Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes.PNAS, 113(13):3521-3526. IF:9.674
•Wang H, Liang L, Dong Q,et al.（2018）Long noncoding RNA miR503HG, a prognostic indicator, inhibits tumor metastasis by regulating the HNRNPA2B1/NF-κB pathway in hepatocellular carcinoma. Theranostics. IF:8.172
相關產品
• 客製化 TaqMan miRNA試劑套組 更多資訊
說明書下載
論文發表
• Liu X ,Wei W ,Li X , et al. (2017). BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3.Gastroenterology. IF:18.392
•Ju J, Chen A, Deng Y, et al. (2017).NatD promotes lung cancer progression by preventing histone H4 serine phosphorylation to activate Slug expression.[J]. Nature Communications, 2017, 8(1):928. IF:12.124
•Ding W, Liu WQ, Jia Y, et al. (2016). Biosynthetic investigation of phomopsins reveals a widespread pathway for ribosomal natural products in Ascomycetes.PNAS, 113(13):3521-3526. IF:9.674
•Wang H, Liang L, Dong Q,et al.（2018）Long noncoding RNA miR503HG, a prognostic indicator, inhibits tumor metastasis by regulating the HNRNPA2B1/NF-κB pathway in hepatocellular carcinoma. Theranostics. IF:8.172
相關產品
• 客製化 TaqMan miRNA試劑套組 更多資訊
說明書下載
檔案下載