(N321/N322) VAHTS Multiplex Oligos Set 4/5 for Illumina
N321/N322
| PN | Product Name | Size | Price (TWD) |
| N321 | VAHTS Multiplex Oligos Set 4 for Illumina | 192 rxn | 35,280 |
| N322 | VAHTS Multiplex Oligos Set5 for Illumina |
192 rxn | 35,280 |
序列訊息
N321_VAHTS Multiplex Oligos Set 4 for Illumina
VAHTS Adapter-S for Illumina
5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
3´-CTGACCTCAAGTCTGCACACGAGAAGGCTAG-p-5´
(-s-表示硫代, -p表示磷酸化)
VAHTS i5 PCR Primers
5´-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
VAHTS i7 PCR Primers
5´-CAAGCAGAAGACGGCATACGAGAT[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´
(-s-表示硫代)
| Sample Sheet輸入/定序時Index序列 | ||||
| VAHTS i5 PCR Primers | i5 Index名稱 | 引子中Index序列 | NovaSeq, MiSeq, HiSeq 2000/2500 |
MiniSeq, NextSeq, HiSeq 3000/4000 |
| DM501 | D501 | TATAGCCT | TATAGCCT | AGGCTATA |
| DM502 | D502 | ATAGAGGC | ATAGAGGC | GCCTCTAT |
| DM503 | D503 | CCTATCCT | CCTATCCT | AGGATAGG |
| DM504 | D504 | GGCTCTGA | GGCTCTGA | TCAGAGCC |
| DM505 | D505 | AGGCGAAG | AGGCGAAG | CTTCGCCT |
| DM506 | D506 | TAATCTTA | TAATCTTA | TAAGATTA |
| DM507 | D507 | CAGGACGT | CAGGACGT | ACGTCCTG |
| DM508 | D508 | GTACTGAC | GTACTGAC | GTCAGTAC |
| VAHTS i7 PCR Primers | i7 Index名稱 | 引子中Index序列 | Sample Sheet輸入/定序時Index序列 |
| DM701 | D701 | CGAGTAAT | ATTACTCG |
| DM702 | D702 | TCTCCGGA | TCCGGAGA |
| DM703 | D703 | AATGAGCG | CGCTCATT |
| DM704 | D704 | GGAATCTC | GAGATTCC |
| DM705 | D705 | TTCTGAAT | ATTCAGAA |
| DM706 | D706 | ACGAATTC | GAATTCGT |
| DM707 | D707 | AGCTTCAG | CTGAAGCT |
| DM708 | D708 | GCGCATTA | TAATGCGC |
| DM709 | D709 | CATAGCCG | CGGCTATG |
| DM710 | D710 | TTCGCGGA | TCCGCGAA |
| DM711 | D711 | GCGCGAGA | TCTCGCGC |
| DM712 | D712 | CTATCGCT | AGCGATAG |
N322_VAHTS Multiplex Oligos Set 5 for Illumina
VAHTS Adapter-S for Illumina
5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
3´-CTGACCTCAAGTCTGCACACGAGAAGGCTAG-p-5´
(-s-表示硫代, -p表示磷酸化)
VAHTS i5 PCR Primers
5´-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC-s-T-3´
VAHTS i7 PCR Primers
5´-CAAGCAGAAGACGGCATACGAGAT[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´
(-s-表示硫代)
| Sample Sheet輸入/定序時Index序列 | ||||
| VAHTS i5 PCR Primers | i5 Index名稱 | 引子中Index序列 | NovaSeq, MiSeq, HiSeq 2000/2500 |
MiniSeq, NextSeq, HiSeq 3000/4000 |
| DM509 | D509 | GACCTGTA | GACCTGTA | TACAGGTC |
| DM510 | D510 | ATGTAACT | ATGTAACT | AGTTACAT |
| DM511 | D511 | GTTTCAGA | GTTTCAGA | TCTGAAAC |
| DM512 | D512 | CACAGGAT | CACAGGAT | ATCCTGTG |
| DM513 | D513 | TAGCTGCC | TAGCTGCC | GGCAGCTA |
| DM514 | D514 | AGCGAATG | AGCGAATG | CATTCGCT |
| DM515 | D515 | TATGCTGC | TATGCTGC | GCAGCATA |
| DM516 | D516 | AGAAGACT | AGAAGACT | AGTCTTCT |
| VAHTS i7 PCR Primers | i7 Index名稱 | 引子中Index序列 | Sample Sheet輸入/定序時Index序列 |
| DM713 | D713 | CCTACACG | CGTGTAGG |
| DM714 | D714 | GTAGTGTC | GACACTAC |
| DM715 | D715 | TGTATGCA | TGCATACA |
| DM716 | D716 | CCAGACTG | CAGTCTGG |
| DM717 | D717 | AGGTGCCA | TGGCACCT |
| DM718 | D718 | TCACCTTG | CAAGGTGA |
| DM719 | D719 | GTATCTTT | AAAGATAC |
| DM720 | D720 | CAGCTCCA | TGGAGCTG |
| DM721 | D721 | TCGCCTTA | TAAGGCGA |
| DM722 | D722 | CTAGTACG | CGTACTAG |
| DM723 | D723 | AGCGTAGC | GCTACGCT |
| DM724 | D724 | GAGCCTCG | CGAGGCTC |
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