COVID-19 qPCR Assay
新冠肺炎核酸檢測試劑
COVID-19 qPCR Product Series
產品特點
* 全球唯一搭配RNA標準品
* COVID-19 Positive Control
* 檢驗RNA Reverse Transcription效率
* 預混式產品減少人工配置的錯誤
* 完整的QC確效
* Performed by 1-step RTqPCR
* 避免偽陽性(false positive)引子探針汙染訊號
* 可單獨訂購各目標基因RNA標準品
* 非DNA標準品只監測PCR的效率
產品種類
qPCR Assay
(Each assay is a 20X validated premix with primers and probe)
| PN | Product Name | Size | Ref | Gene target | NTD |
| 600170 | COVID-19 qPCR Assay (E) | 720uL | WHO | E (Pan COV) |
10,200 |
| 600171 | COVID-19 qPCR Assay (RDRP) | 720uL | WHO | RDRP (COVID-19) |
10,200 |
| 600173 | COVID-19 qPCR Assay (N) | 720uL | WHO | N (Pan COV) |
10,200 |
| 600174 | COVID-19 qPCR Assay (N1) | 720uL | USCDC | N1 (COVID-19) |
10,200 |
| 600175 | COVID-19 qPCR Assay (N2) | 720uL | USCDC | N2 (COVID-19) |
10,200 |
| 600176 | COVID-19 qPCR Assay (N3) | 720uL | USCDC | N3 (COVID-19) |
10,200 |
| 600172 | COVID-19 qPCR Assay (Human Control) |
720uL | USCDC | Human RNase P |
10,200 |
COVID-19 1-Step RTqPCR Kit
| PN | Product Name | Size | Target | NTD |
| COVID19WHO100 | COVID-19 1-step RTqPCR Kit (WHO) | 100rxn | E, RDRP | 31,500 |
| COVID19USCDC100 | COVID-19 1-step RTqPCR Kit (USCDC) | 100rxn | 3 targets in N gene | 42,000 |
COVID-19 1-step qRTPCR Kit Components:
| WHO | USCDC |
| * RdRP gene assay (WHO) | * N1,N2,N3 gene assay (US CDC) |
| * E gene assay (WHO) | * Human RNase P control assay |
| * Human RNase P control assay | * N1,N2,N3 positive control RNA |
| * RdRP positive control RNA | * 1-step RT-qPCR Enzyme/MMIX |
| * E positive control RNA | |
| * 1-step RT-qPCR Enzyme/MMIX |
RNA Standard COVID-19
| PN | Product Name | Size | Ref | NTD |
| RCOVID19WHOE | E | 1E6/100uL | WHO | 7,350 |
| RCOVID19WHORDRP | RDRP | 1E6/100uL | WHO | 7,350 |
| RCOVID19WHON | N | 1E6/100uL | WHO | 7,350 |
| RCOVID19USCDCN1 | N1 | 1E6/100uL | USCDC | 7,350 |
| RCOVID19USCDCN2 | N2 | 1E6/100uL | USCDC | 7,350 |
| RCOVID19USCDCN3 | N3 | 1E6/100uL | USCDC | 7,350 |
1-step qRT-PCR試劑配置表
for Applied Biosystems qPCR Instruments
| Items | μl |
| 2X RT-qPCR Buffer | 5 |
| 20X RT/PCR Enzyme MIX | 0.5 |
| 20X qPCR Assay | 0.5 |
| 50X ROX1/2 | 0.2 |
| Template RNA | 3.8 |
| Total Volume | 10 |
for other qPCR Instruments
| Items | μl |
| 2X RT-qPCR Buffer | 5 |
| 20X RT/PCR Enzyme MIX | 0.5 |
| 20X qPCR Assay | 0.5 |
| Template RNA | 4 |
| Total Volume | 10 |
1-step RT-qPCR 溫度時間循環偵測
| Cycle | Temp | Time | FAM | Note |
| 1 | 50°C | 10 mins | Reverse Transcription | |
| 1 | 95°C | 1 min | ★ Polymerase Activation | |
| 45 | 95°C 58°C |
1 sec 30 secs |
■ | Denature/Anneal/Extend |
■ : Data Collection with FAM fluorescent
(Emission 520nm).
產品說明
RdRP gene (WHO)
N2 gene (USCDC)
E gene (WHO)
Limit of Quantification (LOQ) : 400 copies (100 copies/uL)
參考資料
1. WHO. Diagnostic detection of 2019-nCoV by real-time RT-PCR.
2. US CDC. 2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes