COVID-19 Multiplex 1-step RTqPCR Kit
新冠肺炎核酸多重檢測試劑盒
COVID-19 Multiplex 1-step RTqPCR Kit
產品特點
* 一步式獲得多基因的偵測結果
* 預混式產品減少人工配置的錯誤
* 全球唯一搭配RNA標準品
* COVID-19 Positive Control
* 檢驗RNA Reverse Transcription效率
* 完整的QC確效
* Performed by 1-step RTqPCR
* 避免偽陽性(false positive)引子探針汙染訊號
* 非DNA標準品只監測PCR的效率
產品種類
COVID-19 Multiplex 1-step RTqPCR Kit
| PN | Product Name | Size | Target | NTD |
| COVID19MUL_Rd-N2 | COVID-19 Multiplex 1-Step RTqPCR Kit | 100rxn | RDRP (WHO), N2 (USCDC) |
31,500 |
COVID-19 Multiplex 1-step RTqPCR Kit :
| 1-step Multiplex RT qPCR Enzyme/MMIX | Label |
| * RdRP/N2 gene assay (WHO/USCDC) | RdRP(FAM) / N2(VIC) |
| * Human RNase P control assay * RdRP / N2 / E positive control RNA |
NED |
qRT-PCR試劑配置表
(for All qPCR Instruments)
| Items | μl |
| 4X RdRP/N2 MMIX | 2.5 |
| ddH2O | 3.5 |
| RNA sample | 4 |
| Total Volume | 10 |
| Items | μl |
| 4X Human RNase P MMIX | 2.5 |
| ddH2O | 3.5 |
| RNA sample | 4 |
| Total Volume | 10 |
1-step RT-qPCR 溫度時間循環偵測
| Cycle | Temp | Time | FAM/VIC | Note |
| 1 | 50°C | 10 mins | Reverse Transcription | |
| 1 | 95°C | 1 min | ★ Polymerase Activation | |
| 45 | 95°C 58°C |
1 sec 30 secs |
■ | Denature/Anneal/Extend |
■ : Data Collection with FAM/VIC fluorescent (Emission 520/550nm).
產品說明
RdRP gene (WHO)
N2 Gene (USCDC)
Limit of Quantification (LOQ) : 20 copies (100 copies/uL)
參考資料
1. WHO. Diagnostic detection of 2019-nCoV by real-time RT-PCR.
2. US CDC. 2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes