TopQ Circular RNA (circRNA) Probe qPCR Assay
603002
| PN | Product Name | Size | Price (TWD) |
| 603002 | TopQ CircRNA Probe qPCR Assay | 250 µL (500 rxns) |
5,250 |
| 組分 | |
| 20X TopQ CircRNA Probe qPCR Assay | 250 µL (500 rxns) |
| CircRNA cDNA Standard (Positive Control) |
10 µL (10^2/µL) |
反轉錄試劑建議使用
HiScript III RT SuperMix for qPCR #R323 (含Random RT primer)
TaqMan專用預混液建議使用
TopGE Probe Fast qPCR Master Mix #TGE
簡介
Circular RNA (circRNA)是一種單鏈、環狀的RNA，類似長鏈的non-coding RNA，其調控機制與microRNA (miRNA)相似
原理說明
1. 利用real-time PCR (qPCR) 方式並配合探針 (TaqMan Probe) 鑑別 Unique circular RNA junction (Figure 1)
2. TopQ circRNA probe qPCR法可準確偵測目標 spliced circular RNA length (Figure 2)
1. 利用real-time PCR (qPCR) 方式並配合探針 (TaqMan Probe) 鑑別 Unique circular RNA junction (Figure 1)
2. TopQ circRNA probe qPCR法可準確偵測目標 spliced circular RNA length (Figure 2)
3. SYBR Green qPCR法易受到其他spliced circRNA干擾 (Figure 2)
訂購方式
需依據circBase資料庫提供物種的 circBase ID (ex: hsa_circ_0018046)
*circBase連結: www.circbase.org
*circBank連結: www.circbank.cn
| NO. | circRNA | circRNA length (spliced) | Host gene Symbol |
| 1 | hsa_circ_0000234 | 339 | CUL2 [註1] |
| 2 | |||
| 3 |
[註1]
參考文獻
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7643398/pdf/12943_2020_Article_1270.pdf
• HiScript III RT SuperMix for qPCR #R323 (建議 circular RNA (circRNA) 反轉錄使用)
• TopGE Probe Fast qPCR Master Mix #TGE (建議 circular RNA (circRNA) qPCR 偵測使用)
庫存清單
• TopGE Probe Fast qPCR Master Mix #TGE (建議 circular RNA (circRNA) qPCR 偵測使用)
庫存清單
| Human | Others |
|---|---|
| hsa_circ_0000234 (circ_CUL2) | |
| hsa_circ_0007142 (circ_DOCK1) | |
| hsa_circ_0021776 (circ_TTC17) |