COVID-19 Alpha qPCR Assay
新冠肺炎Alpha核酸檢測試劑
COVID-19 Alpha qPCR Assay (SARS-CoV-2 B.1.1.7)
產品特點
* 準確偵測 SARS-CoV-2 B.1.1.7 strain (Alpha)
* 目標 : Orf1ab gene A1708D區段
* 全球唯一搭配 RNA 陽性標準品
* Alpha Positive RNA Control (Orf1ab gene A1708D)
* 可檢驗 RNA Reverse Transcription效率
* 預混式產品減少人工配置的錯誤
* 完整的QC確效
* Performed by 1-step RTqPCR
* 避免偽陽性 (False Positive) 引子探針汙染訊號
* 非 DNA標準品 只監測PCR的效率
產品種類
qPCR Assay (20X Alpha qPCR assay, PC RNA)
(Each assay is a 20X validated premix with primers and probe)
| PN | Product Name | Size | Ref | Gene target |
NTD |
| 600185 | COVID-19 Alpha qPCR Assay | 720uL | GISAID | Orf1ab (A1708D) |
10,200 |
COVID-19 1-Step RTqPCR Kit (including 1-Step RTqPCR MMIX, PC RNA)
| PN | Product Name | Size | Target | NTD |
| COVIDB117 | COVID-19 1-step RTqPCR Kit (Alpha) | 100rxn | Orf1ab (A1708D) |
31,500 |
COVID-19 1-step qRTPCR Kit
| Components |
| * 20X Orf1ab A1708D assay (GISAID) |
| * Positive control RNA 1E6/uL, 100uL |
| * 4X 1step RTqPCR MMIX |
RNA Standard COVID-19 Alpha Orf1ab+A1708D
| PN | Product Name | Size | Ref | NTD |
| RCOVID19ALPO | Orf1ab+A1708D | 1E6/uL, 100uL | GISAID | 7,350 |
1-step qRT-PCR試劑配置表
for Applied Biosystems qPCR Instruments
| Items | μl |
| H2O | 3 |
| 4X 1stepRTqPCR MMIX | 2.5 |
| 20X Alpha qPCR Assay | 0.5 |
| Template RNA | 4 |
| Total Volume | 10 |
1-step RTqPCR 溫度時間循環偵測
| Cycle | Temp | Time | FAM | Note |
| 1 | 50°C | 10 mins | Reverse Transcription | |
| 1 | 95°C | 1 min | ★ Polymerase Activation | |
| 45 | 95°C 58°C |
1 sec 30 secs |
■ | Denature/Anneal/Extend |
■ : Data Collection with FAM fluorescent
(Emission 520nm).
產品說明
Spike R214+EPEins gene (GISAID)
Limit of Detection (LOD) : 40 copies (10 copies/uL)
Limit of Quantification (LOQ) : 400 copies (100 copies/uL)