Protease Inhibitor Cocktail (EDTA-free) 蛋白酶抑制劑
R4693132001
Protease Inhibitor Cocktail (EDTA-free) 蛋白酶抑制劑
Research Use Only Not for Diagnostic Intended
科學研究用非提供臨床診斷使用
Tablets provided in EASY packs
每錠獨立分離 採用可視型鋁箔包裝 確保防潮與污染
適合用在
* 蛋白質純化分離
* 蛋白西方墨點法分析
* DNA蛋白免疫沉澱實驗
# Western Blot
# RIPA Buffer
# Immunoprecipitation IP
# Cut and Tag
# ChIP
使用大廠原料相同成分
可參考
sigmaaldrich.com/TW/en/product/roche/04693132001
保存溫度
> Tablets stored at +2 to +8°C.
> Store stock at +4°C for up to 2 wks
or −20°C for at least 3 months.
配置方式說明
Preparation of
50X-concentrated stock solution:
Dissolve one Protease inhibitor (EDTA-free) tablet
in 1 ml double-distilled water
or in 1ml 100 mM phosphate buffer, pH 7.0.
| PN | Product Name | Size | Price (TWD) |
| R4693132001-T5 | Protease Inhibitor Cocktail (EDTA-free) 蛋白酶抑制劑 |
5錠 | 7,750 |
| R4693132001-T10 | Protease Inhibitor Cocktail (EDTA-free) 蛋白酶抑制劑 |
10錠 | 12,600 |
Research Use Only Not for Diagnostic Intended
科學研究用非提供臨床診斷使用
Tablets provided in EASY packs
每錠獨立分離 採用可視型鋁箔包裝 確保防潮與污染
適合用在
* 蛋白質純化分離
* 蛋白西方墨點法分析
* DNA蛋白免疫沉澱實驗
Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903)
CUT & Tag NGS Sequencing and Bioinformatic Analysis
CUT & Tag NGS Sequencing and Bioinformatic Analysis
# Western Blot
# RIPA Buffer
# Immunoprecipitation IP
# Cut and Tag
# ChIP
使用大廠原料相同成分
可參考
sigmaaldrich.com/TW/en/product/roche/04693132001
保存溫度
> Tablets stored at +2 to +8°C.
> Store stock at +4°C for up to 2 wks
or −20°C for at least 3 months.
配置方式說明
Preparation of
50X-concentrated stock solution:
Dissolve one Protease inhibitor (EDTA-free) tablet
in 1 ml double-distilled water
or in 1ml 100 mM phosphate buffer, pH 7.0.
General Description
Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains.
Due to the optimized composition of the tablets, they show excellent inhibition effects,
and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
Tablets contain EDTA and both irreversible and reversible protease inhibitors.
Protease inhibitors present in Tablets do not form irreversible complexes with the SH groups of proteins.
Due to the optimized composition of the tablets, they show excellent inhibition effects,
and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
Tablets contain EDTA and both irreversible and reversible protease inhibitors.
Protease inhibitors present in Tablets do not form irreversible complexes with the SH groups of proteins.
Specificity
Inhibits a broad spectrum of serine proteases, cysteine proteases, and metalloproteases, as well as calpains.
Application
Protease Inhibitor Cocktail has been used as an component in
- extraction buffer for protein extraction from Arabidopsis seedling
- in radioimmunoprecipitation assay (RIPA) buffer for the isolation of protein from lymphocytes
- the extraction buffer for the lysis of protoplast.
Protease Inhibitor Cocktail is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
It contains both reversible and irreversible protease inhibitors.
Therefore, we recommend the addition of product to all stock buffers and solutions normally protected with protease inhibitors,
and not just during the initial purification steps.
It contains both reversible and irreversible protease inhibitors.
Therefore, we recommend the addition of product to all stock buffers and solutions normally protected with protease inhibitors,
and not just during the initial purification steps.
Features and Benefits
Use Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.
- Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASY pack into your buffer.
- Complete protection: Instantly protect your proteins against a broad range of proteases.
- Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
- Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.