TruePrep DNA Library Prep Kit V2 for Illumina
TD501/TD502/TD503
|
PN
|
Product Name
|
Size
|
Price (TWD)
|
|
TD501-01
|
TruePrep DNA Library Prep
Kit V2 for Illumina (50ng) |
24 rxn
|
41,370
|
|
TD501-02
|
TruePrep DNA Library Prep Kit V2 for Illumina (50ng) |
96 rxn
|
159,810
|
|
TD502-01
|
TruePrep DNA Library Prep
Kit V2 for Illumina (5ng) |
24 rxn
|
41,370
|
|
TD502-02
|
TruePrep DNA Library Prep
Kit V2 for Illumina (5ng) |
96 rxn
|
159,810
|
|
TD503-01
|
TruePrep DNA Library Prep Kit V2 for Illumina (1ng) |
24 rxn
|
41,370
|
|
TD503-02
|
TruePrep DNA Library Prep
Kit V2 for Illumina (1ng) |
96 rxn
|
159,810
|
優勢
• 單個樣品建庫時間少於90分鐘
• 單管酶促反應，片段化和加接頭反應同時進行，無需使用機械方法(超聲、霧化等)進行片段化
• 文庫擴增專用聚合酶，配以精心優化的反應緩衝，最大程度上保證了文庫擴增的高效性和均一性
• 建庫起始DNA量最低僅需1 ng
• 根據起始DNA量分類，提供共計3種不同規格試劑盒
• 提供96種雙端index組合
說明
TruePrep DNA Library Prep Kit V2 for Illumina是針對Illumina高通量測序平台定向開發的專用試劑盒。使用該試劑盒可以將DNA製備成Illumina高通量測序平台專用測序文庫。和傳統文庫構建方法相比，TruePrepTM試劑盒採用新型的轉座酶法進行DNA片段化，將繁瑣的DNA片段化、末端修復和接頭連接反應等步驟變為一步簡單的酶促反應，顯著降低了起始DNA的需求量並縮短了文庫構建時間。試劑盒共提供50 ng、 5 ng、 1 ng三種規格，可根據實驗類型自由選擇。試劑盒中提供的所有試劑都經過嚴格的質量控制和功能驗證，最大程度上保證了文庫構建的穩定性和重複性。
Mechanism of Vazyme's TruePrep Series
TruePrep Tagment Enzyme Mix (TTE Mix) contains transposase and two kinds of adapters (Adapter 1 and Adapter 2) with equal molar. Input DNA are fragmented and linked with adapters on both ends just by mixing with TTE Mix, followed by a 10-minute incubation at 55°C. The tagged DNA fragments can be further amplified with two pairs of primers N5 (N5XX) / N7 (N7XX) and P5 / P7 (PCR Primer Mix, PPM). After size selection and purification, the library is ready for sequencing on Illumina platforms.
Adapter 1 and Adapter 2, two oligos embedded in TruePrep Tagment Enzyme
P5 and P7, two universal PCR Primers
N5 and N7, two index primers containing index 2 (i5) and index 1 (i7) respectively
• 單管酶促反應，片段化和加接頭反應同時進行，無需使用機械方法(超聲、霧化等)進行片段化
• 文庫擴增專用聚合酶，配以精心優化的反應緩衝，最大程度上保證了文庫擴增的高效性和均一性
• 建庫起始DNA量最低僅需1 ng
• 根據起始DNA量分類，提供共計3種不同規格試劑盒
• 提供96種雙端index組合
說明
TruePrep DNA Library Prep Kit V2 for Illumina是針對Illumina高通量測序平台定向開發的專用試劑盒。使用該試劑盒可以將DNA製備成Illumina高通量測序平台專用測序文庫。和傳統文庫構建方法相比，TruePrepTM試劑盒採用新型的轉座酶法進行DNA片段化，將繁瑣的DNA片段化、末端修復和接頭連接反應等步驟變為一步簡單的酶促反應，顯著降低了起始DNA的需求量並縮短了文庫構建時間。試劑盒共提供50 ng、 5 ng、 1 ng三種規格，可根據實驗類型自由選擇。試劑盒中提供的所有試劑都經過嚴格的質量控制和功能驗證，最大程度上保證了文庫構建的穩定性和重複性。
Mechanism of Vazyme's TruePrep Series
TruePrep Tagment Enzyme Mix (TTE Mix) contains transposase and two kinds of adapters (Adapter 1 and Adapter 2) with equal molar. Input DNA are fragmented and linked with adapters on both ends just by mixing with TTE Mix, followed by a 10-minute incubation at 55°C. The tagged DNA fragments can be further amplified with two pairs of primers N5 (N5XX) / N7 (N7XX) and P5 / P7 (PCR Primer Mix, PPM). After size selection and purification, the library is ready for sequencing on Illumina platforms.
P5 and P7, two universal PCR Primers
N5 and N7, two index primers containing index 2 (i5) and index 1 (i7) respectively
實驗案例
TruePrep超快速建庫流程與傳統建庫流程耗時對比。 TruePrep建庫系統將繁瑣的DNA處理流程簡化為一步10 min作用，將單個文庫構建時間縮短至90 min之內，使得單人單日可以達到96個文庫的建庫通量。
期刊發表
Zhang, Lei, et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature. 2018 IF: 41.577
** Vazyme轉座酶建庫產品首登神經學頂級期刊《Neuron》新聞連結
說明書下載
TruePrep超快速建庫流程與傳統建庫流程耗時對比。 TruePrep建庫系統將繁瑣的DNA處理流程簡化為一步10 min作用，將單個文庫構建時間縮短至90 min之內，使得單人單日可以達到96個文庫的建庫通量。
期刊發表
Zhang, Lei, et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature. 2018 IF: 41.577
** Vazyme轉座酶建庫產品首登神經學頂級期刊《Neuron》新聞連結
說明書下載
檔案下載