TopQ miRNA Probe qPCR Assay
602001
PN | Product Name | Size | Price (TWD) |
602001 | TopQ miRNA Probe qPCR Assay |
250ul (500rxns) |
8,400 |
組分 | |
20X TaqMan miRNA Assay | 250 µl (500 rxns) |
20X miRNA RT Primer | 200 µl (200 rxns) |
miRNA Positive Control | 100 µl (1E5 Copies/µl) |
● 市面上唯一提供miRNA標準品 確保實驗準確無誤!
● 實驗特點:
- Step 1 : Reverse Transcription
- Step 2 : Real-time PCR reaction
庫存清單
Human |
---|
hsa-let-7a-5p |
hsa-let-7b-5p |
hsa-let-7c-5p |
hsa-let-7i-5p |
hsa-miR-101-3p |
hsa-miR-10a-5p |
hsa-miR-10b-5p |
hsa-miR-11181-5p |
hsa-miR-122-5p |
hsa-miR-1246 |
hsa-miR-125a-5p |
hsa-miR-125b-5p |
hsa-miR-126-3p |
hsa-miR-126-5p |
hsa-miR-1290 |
hsa-miR-130a-5p |
hsa-miR-132-3p |
hsa-miR-133a-3p |
hsa-miR-133b |
hsa-miR-134-5p |
hsa-miR-136-3p |
hsa-miR-137-3p |
hsa-miR-140-5p |
hsa-miR-141-3p |
hsa-miR-142-3p |
hsa-miR-143-3p |
hsa-miR-145-5p |
hsa-miR-146a-5p |
hsa-miR-146b-5p |
hsa-miR-147b-3p |
hsa-miR-148a-3p |
hsa-miR-148b-3p |
hsa-miR-149-3p |
hsa-miR-149-5p |
hsa-miR-155-5p |
hsa-miR-15a-5p |
hsa-miR-16-5p |
hsa-miR-17-3p |
hsa-miR-17-5p |
hsa-miR-181a-5p |
hsa-miR-181b-5p |
hsa-miR-181c-5p |
hsa-miR-182-3p |
hsa-miR-182-5p |
hsa-miR-183-5p |
hsa-miR-184 |
hsa-miR-187-3p |
hsa-miR-18a-5p |
hsa-miR-191-5p |
hsa-miR-192-5p |
hsa-miR-193a-5p |
hsa-miR-193b-3p |
hsa-miR-194-3p |
hsa-miR-194-5p |
hsa-miR-195-5p |
hsa-miR-196a-5p |
hsa-miR-196b-3p |
hsa-miR-196b-5p |
hsa-miR-1972 |
hsa-miR-199a-3p |
hsa-miR-199a-5p |
hsa-miR-19a-3p |
hsa-miR-19b-3p |
hsa-miR-1-3p |
hsa-miR-200a-3p |
hsa-miR-200b-3p |
hsa-miR-200c-3p |
hsa-miR-206 |
hsa-miR-20a-5p |
hsa-miR-210-3p |
hsa-miR-211-5p |
hsa-miR-212-3p |
hsa-miR-214-3p |
hsa-miR-216a-5p |
hsa-miR-21-5p |
hsa-miR-218-5p |
hsa-miR-221-3p |
hsa-miR-221-5p |
hsa-miR-222-3p |
hsa-miR-22-3p |
hsa-miR-224-5p |
hsa-miR-23a-3p |
hsa-miR-23b-3p |
hsa-miR-26a-5p |
hsa-miR-27b-3p |
hsa-miR-296-3p |
hsa-miR-299-5p |
hsa-miR-29a-3p |
hsa-miR-29a-5p |
hsa-miR-29b-3p |
hsa-miR-29c-3p |
hsa-miR-301a-3p |
hsa-miR-301b-3p |
hsa-miR-3059-3p |
hsa-miR-30a-5p |
hsa-miR-30c-5p |
hsa-miR-30d-5p |
hsa-miR-30e-5p |
hsa-miR-31-3p |
hsa-miR-31-5p |
hsa-miR-3130-5p |
hsa-miR-3177-5p |
hsa-miR-3194-5p |
hsa-miR-320a-3p |
hsa-miR-320d |
hsa-miR-330-3p |
hsa-miR-331-3p |
hsa-miR-335-3p |
hsa-miR-33b-5p |
hsa-miR-342-3p |
hsa-miR-361-5p |
hsa-miR-3620-5p |
hsa-miR-363-5p |
hsa-miR-3663-3p |
hsa-miR-370-3p |
hsa-miR-372-3p |
hsa-miR-373-3p |
hsa-miR-374b-5p |
hsa-miR-375-3p |
hsa-miR-379-5p |
hsa-miR-409-3p |
hsa-miR-409-5p |
hsa-miR-423-5p |
hsa-miR-425-5p |
hsa-miR-4443 |
hsa-miR-4454 |
hsa-miR-449a |
hsa-miR-451a |
hsa-miR-4516 |
hsa-miR-4652-5p |
hsa-miR-4669 |
hsa-miR-483-3p |
hsa-miR-485-3p |
hsa-miR-483-5p |
hsa-miR-486-5p |
hsa-miR-487b-3p |
hsa-miR-491-5p |
hsa-miR-494-5p |
hsa-miR-495-3p |
hsa-miR-5010-3p |
hsa-miR-502-5p |
hsa-miR-505-3p |
hsa-miR-516a-5p |
hsa-miR-518d-3p |
hsa-miR-519a-3p |
hsa-miR-520b-3p |
hsa-miR-524-5p |
hsa-miR-532-5p |
hsa-miR-539-5p |
hsa-miR-543 |
hsa-miR-545-3p |
hsa-miR-548ah-3p |
hsa-miR-548av-3p |
hsa-miR-589-5p |
hsa-miR-6503-3p |
hsa-miR-652-5p |
hsa-miR-708-3p |
hsa-miR-7-1-3p |
hsa-miR-7-5p |
hsa-miR-92a-3p |
hsa-miR-92b-3p |
hsa-miR-96-5p |
Others |
---|
cel-miR-39-3p |
mmu-let-7k |
mmu-miR-1195 |
mmu-miR-1198-5p |
mmu-miR-124-3p |
mmu-miR-129-5p |
mmu-miR-144-5p |
rno-miR-146b-5p |
mmu-miR-152-3p |
mmu-miR-155-5p |
mmu-miR-204-5p |
mmu-miR-211-5p |
mmu-miR-223-3p |
mmu-miR-24-2-5p |
mmu-miR-24-3p |
mmu-miR-34a-5p |
mmu-miR-350-3p |
mmu-miR-3535 |
mmu-miR-362-3p |
mmu-miR-434-3p |
mmu-miR-483-3p |
mmu-miR-505-3p |
rno-miR-146b-5p |
rno-miR-223-3p |
rno-miR-324-3p |
rno-miR-3584-5p |
rno-miR-378b |
rno-miR-93-5p |
U6 snRNA (Human/ Mouse/ Rat) |
4.5s RNA (Mouse/ Rat) |
說明
● microRNA為19-22nt的短的非编碼RNA。
● 與mRNA的3'-UTR區域結合,進而在轉錄後調控mRNA的表達。
● 在不同物種中,miRNA 序列具有高度的保守性 ( Conserved ),具有組織特異性。
● microRNA的表達改變與目前各種已知疾病相關。
原理
● 採用廣泛認可的Stem-loop法,其包含miRNA特異反轉錄引物以及qPCR正、反向引子。
環結構狀的miRNA特異反轉錄引物可特異性的轉錄mature miRNA。再利用高度特異的正反向引子,使miRNA定量既靈敏又具高度特異。
● 提供miRNA spike-in,以監控miRNA純化過程是否有較大損失和反轉錄與定量PCR是否有PCR抑制劑。
實驗範例
miRNA檢測範例
圖1. qPCR檢測MCF Cell 中miRNA-21-5p的表達情況
*miRBase連結: www.mirbase.org/
NO. | miRNA | Accession | Species | 備註 |
0 | hsa-miR-21-5p | MIMAT0000076 | Human | 範例 |
1 | ||||
2 |
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