人類細胞型態鑑別
Human Cell Lineage Identification
相關應用
鑑別方式
一. NGS
二. qPCR Array
NGS
透過化學法將RNA片斷化後、建文庫，再通過新一代高通量定序，能夠全面快速的獲人類特定組織或細胞在某一狀態下的幾乎所有轉錄本序列訊息，進行序列比對後，來鑑別細胞型態。
相關資訊參考連結: Link
QPCR Array
參考資料
Qiagen QPCR Array Cell Lineage Identification (PAHS-508Z) Link
相關應用
- 自行培養細胞
- 胚層來源
- 分化型態
- 快速基因分類鑑別
鑑別方式
一. NGS
二. qPCR Array
NGS
透過化學法將RNA片斷化後、建文庫，再通過新一代高通量定序，能夠全面快速的獲人類特定組織或細胞在某一狀態下的幾乎所有轉錄本序列訊息，進行序列比對後，來鑑別細胞型態。
相關資訊參考連結: Link
QPCR Array
- 技術流程
- Array Makers list:
|
Pluripotency Markers
|
|
|
Pluripotency Markers
|
DNMT3B,GDF3 (VGR-2),LEFTY1,NANOG,PODXL, POU5F1 (OCT4),ZFP42
|
|
Germ Layers
|
|
|
Ectoderm
|
FGF5,FOXD3,OTX2,ZIC1
|
|
Neuroectoderm
|
GBX2,NEUROG2
|
|
Mesoderm
|
BMP4,CD34,DCN,GATA2,
HAND1,IGF2,MIXL1,PDGFRA, RUNX1 (AML1),TBXT (Brachyury) |
|
Endoderm
|
FOXA1,GATA1,GATA6,HNF4A,
SOX17,SOX7 |
|
Ectoderm Progenitors
|
|
|
Neuronal Progenitors
|
FABP7,HES5,PROM1,SOX2
|
|
Immature Neurons
|
DCX
|
|
Immature GABA Neurons
|
GAD2,SLC32A1
|
|
Limbal Progenitors
|
ENO1,MSLN
|
|
Motor Neuron Progenitors
|
FOXG1,OLIG2
|
|
Oligodendrocyte Progenitors
|
NKX2-2,OLIG2
|
|
Mesoderm Progenitors
|
|
|
Early Cardiomyocytes
|
HAND2
|
|
Early B Cells
|
CD79A
|
|
Early T Cells
|
CD3E,PTCRA
|
|
Muscle Stem Cells
|
CD34
|
|
Endoderm Progenitors
|
|
|
Pancreatic Islet Cells
|
KRT19
|
|
Hepatic Stem Cells
|
APOH,DPP4,MAP3K12
|
|
Ectoderm Terminal Differentiation Markers
|
|
|
Keratinocytes
|
KRT10,KRT14
|
|
Melanocytes
|
TYR
|
|
Mature Neurons
|
NEUROD1
|
|
Cholinergic Neurons
|
CHAT
|
|
GABA Neurons
|
GAD1
|
|
Glutamatergic Neurons
|
SLC17A6,SLC17A7
|
|
Astrocytes
|
GALC,GFAP
|
|
Ganglion Cells
|
POU4F2
|
|
Photoreceptor Cells
|
RCVRN
|
|
Mesoderm Terminal Differentiation Markers
|
|
|
Skeletal Muscle Cells
|
MYH1
|
|
Smooth Muscle Cells
|
MYH11,SMTN
|
|
Cardiomyocytes
|
MYH7,MYL3,NPPA,RYR2
|
|
Osteoblasts
|
IBSP
|
|
Osteoclasts
|
CTSK
|
|
Chondrocytes
|
COL10A1,COMP
|
|
Macrophages
|
CCR5
|
|
Endoderm Terminal Differentiation Markers
|
|
|
Hepatocytes
|
ALB,G6PC,TAT
|
|
Cholangiocytes
|
ITGB4
|
|
Beta Cells
|
INS,SLC2A2
|
|
Exocrine Cells
|
CPA1
|
|
Lung Cells
|
SFTPB,SFTPD
|
|
Proximal Tubule Cells
|
AQP1,MIOX
|
參考資料
Qiagen QPCR Array Cell Lineage Identification (PAHS-508Z) Link